1g of soil in 6mL of LB media after being shaken in a shaker machine for one week. Chunks of soil are now broken up and settled on the bottom of the tube with a dark yellow/light brown liquid above it.
1g of soil in 6mL of LB media
Sample of soil was collected from cemetery
The soil was collected at a water site near Greens Bayou.
Before the temple was constructed, the area was flooded by Harvey Hurricane and the soil might contain potential bacteria that possess desirable strains.
dirt spot: (29.7617512, -95.3861573)
The sample was collected after a rainy day from in front of a sewer grate. The sample was consistency similar to clay and was somewhat wet at the time of collection.
The sample on a field located next to a restaurant, but in proximity to the main part of Discovery Green. Paraoxon colonies could not be counted on the plate, due to overgrowth. The sample is negative for Methyl Parathion.
Collected 8/29/2018, 7:30 AM. Soil sample was collected nearby bayou and construction site by a biking trail. Soil sample shows negative for Paraoxon under GGM- PX assay.
1,256 colony count.
Sample is able to degrade to OP compound known as Px.
Located near the edge of Brays Bayou, sample was collected around 8:35 on the morning of the 10th of September, 2018.
Yellow coloration around some bacterial colonies indicates some bacterial hydrolysis of the pesticide paraoxon.
Located by the fountain
Sample collected from: Northwest Houston from the soil near the outskirts of a retention pond.
Coordinates 29.988894, -95.475892
Colonies were tolerant when grown in PX GMM Plate, but showed no sign of OP degradation.
Sample was collected from a creek bed at a residential park.
The sample was collected by the Brays Bayou near the University of Houston.
Sample was taken from riverbed at Buffalo Bayou Park. Location was 15meters from a common running path. The site had indications that water levels were subject to constant change according to the weather. Soil sample was mostly sandlike in texture and fairly moist. Site was located under a bridge.
Tolerant of Paraoxon but not able to degrade it, and intolerable of methyl parathion. A gross colony count of around 615.
Soil Sample from Keegans Bayou
When collected, the soil sample had a dark muddy appearance.
The sample was collected at River Terrace park in Channelview Texas. The GMM plate was negative for Px degradation.
the sample was collected from my backyard
Sample was taken along the ditch line, about 8 - 12 inches deep
I had a colony overgrowth.
I had colony growth with a few cells that had Halos, indicative of OP degradation.
Sample found near a neighborhood man made pond.
The collected soil sample that I have provided was obtained at the coordinates 29.7238200, -95.1137860 on August 28, 2018 at 12:41PM on a sunny day. The collected sample was taken from the East Fork Patrick Bayou nearby the Buffalo Bayou. The sample was retrieved from an 8-12 inch hole along the bank of the bayou. For week 0, 1.05g of the sample is initially inoculated into an enriched media then for week 1-5 200µL of the previous week’s inoculation culture is added to separate minimal media tubes containing Carbon Selective Media with Paraoxon, Carbon Selective Media with Methyl Parathion, Sulfur Selective Media with Paraoxon, and Sulfur Selective Media with Methyl Parathion. The best indicator for the metabolism of pesticides is then inoculated onto the enriched media for bacteria revitalization then 100μL of the enriched media with bacteria is plated onto a minimal media with MP. The plated sample is then analyzed to confirm that the bacteria is able to tolerate the Methyl Parathion compounds, but not degrade it.
Outside of a neighborhood, in a big field with long grass and a tunnel water pipe running through it.
The sample was collected in Houston Texas.
My sample was collected on August 26th, in League City, TX 77573.
3 feet from the river
The location the soil sample was taken from a vegetable farm at 3217 Spring Cypress Rd, Cypress TX 77388.
An 8-12 inch deep was dug at this location and about 10g sample was taken. It was stored in a container that protected the sample from UV sunlight. In addition the surrounding temperature was kept at room temperature.
Sample was collected from a garden that is regularly treated with pesticide. However, there was no growth on the plates.
My sample was very moist prior to inoculation. My bacteria was plated on CSM agar with Paraoxon and developed no significant growth.
Collected from the backyard of my home.
My soil sample was collected from my backyard. We occasionally treat for insects. The bacteria in the sample was paraoxon positive and methyl parathion negative.
Sample was taken from a backyard in a residential home in Houston. The sample was positive for Paraoxon tolerance and was able to degrade the pesticide and OP compounds.
Sample collected in a residential area.
Soil Sample was collected from Atkinson farm that grows strawberries. No growth of bacteria was observed on minimal agar plate. The bacteria sampled were not able to withstand or tolerate the pesticides.
Soil Sample taken from a residential backyard garden in Friendswood, Texas, which is maintained by the owner to grow various vegetables and spices.
Methyl parathion and paraoxon - negative results for all inoculation weeks. Paraoxon Methyl parathion CSM PSM SSM CSM PSM SSM Week 1 N N N N N N Week 2 W W W W W W Week 3 T T T T T T Week 4 T T T T T T Week 5 T T T T T T
This sample was obtained at the bayou that was located at the intersection off of grandparkway (SH 99) and FM 1464 in Sugar Land, Texas.
The sample degraded Paraoxon and used it for a carbon source and a sulfur source. It showed no signs of being able to degrade Methyl Parathion. It did seem slow to adapt to relying solely on the pesticide as a food souce, but once it adjusted it became every adept at it.
Halo was present surrounding Bacterial colonies in CSM- Px Agar plate indicating bacteria was able to hydrolyze organophosphorus compound and giving a positive result.
I collected my sample from a corn maze, the ground was wet due to raining. I dug ground about 6-7 inches and collected sample. I stored sample in a dark tube and kept it in mild environment.
Dirt was collect at a cotton farm by hwy 99 at the corner of morton rd and harlem rd in richmond tx at 7:30pm
The soil sample was black, and it was collected from a cotton field on September 1st, 2014. It tested positive for paraoxon and negative for methyl parathion. After ten days of incubation, it was observed too many to count (TMTC) colonies on the minimal agar plates. However, I counted approximately 22,069 colonies.
Soil Sample was collected from cotton field on Benton Rd in Richmond, Texas
No significant color changes were found after 5 weeks of inoculation in Methyl Parathion and Paraoxon.Sample was taken 5 meters into cotton field immediately northwest of the Student Parking Lot.
Very small colonies, non-clustered, white in color, numbering around 80 total. No immediate signs of clearing or degradation to indicate Paraoxon hydrolysis; however, there is sufficient evidence of growth.
Sample displayed tolerance for Methyl parathion on CSM.
soil sample, texture was very fine
Soil sample was taken from Froberg Farm on August 30th, 2014 at around 11 am. At the time when soil sample was taken, no produce were seen growing on site. The farm land was being prepared for planting new batch of seeds. After the weekly inoculation, an average of 19 colonies were seen growing on the agar plate. The bacteria are tolerant to withstand the toxic environment however they are unable to degrade the organophosphorus compound present in the pesticide
The soil was taken on September 1, 2014 around 12pm. The soil was a little wet due to the raining the previous day. The soil was gathered on the right side of the farm when coming in from the parking lot. CSM- organophosphorus is used at carbon source for bacteria culture/growth SSM- organophosphorus is used at sulfur source for bacteria culture/growth Px - Paraoxon MP- Methyl Parathion Inoculations by week Week 1 CSM (Px): light brown and turbid SSM (Px): soil color CSM (MP): light brown and turbid CSM (MP): soil color Week 2 CSM (Px): slight yellow and turbid SSM (Px): slight yellow and turbid CSM (MP): slight yellow and turbid SSM (MP): slight yellow and turbid Week 3 CSM (Px): yellow and clear SSM (Px): colorless CSM (MP): colorless SSM (MP): colorless Week 4 CSM (Px): yellow and clear SSM (Px): colorless CSM (MP): colorless SSM (MP): colorless Week 5 CSM (Px): yellow and clear SSM (Px): colorless CSM (MP): slight yellow and clear SSM (MP): colorless Result There was no culture growth observed in enriched media so minimal media agar was not performed.
The soil sample was taken from Froberg's Farm, where pesticides are used. The bacteria from the Week 5 CSM+Px test tube were able to tolerate Px but not degrade it. The growth on the plate was very weak, resulting in approximately 20,208 colonies.
Paraoxon (PX) Methyl Parathion (MP) CSM SSM CSM SSM Week 1 So So So So Week 2 T T T T Week 3 T T T T Week 4 T T S S Week 5 Y T S S Negative results for both Paraxoan and Methyl Parathion degrading bacteria.
Sample was taken from a small floral garden. Soil was dry, light brown in color, and full of small rocks.
Soil collected from median lane between two driveways located near the building of MD Anderson Cancer Center Proton Therapy Center on the street of Old Spanish Trail, Houston, TX
The soil bacteria sample was collected from the Japanese garden within Hermann park.
a small soil sample was collected from the Mint beds in the botanical garden across from Miller Outdoor theater.
Tommy Lo Week 1 Paraoxon Methyl Parathion CSM PSM SSM CSM PSM SSM N N N N N N Week 2 W W W W W W Week 3 T T T T T T Week 4 T T S T T T Week 5 S S C W T T
The area where the sample was obtained is known to have their course exposed to pesticides in order to keep the grass healthy. However no results where obtained at all. This could have to do with the that part of the golf course might be new or the course isn't as exposed to pesticides as one would think.
A soil sample collected from a grass farm in Crosby Texas.
Soiled sample collected from a field used to grow vegetables on Stuebner Airline Rd.
i have collected a soil sample from a flowerbed located in the Gulfgate (620 and I45s)
It was collected in farm on the country side of Bandera county, San Antonio, Texas. The soil sample was collected from a organic farm where they grow tomatoes, chilies, etc. The soil was dry and hard to found a moist collection. The hole were the soil was collected was about 8cm deep. Sample was taken to the lab 4 days after collection. It was kept in room temperature all the time.
The overall soil sample was dry, but there were some damp soil. The soil was inoculated over a period of 5 weeks. The overall test results were all negative
Sample was collected on a day after which it had rained.
This sample was collected from a creek in Cypress, TX next to farmland. I chose this location due to the vast amount of farmland in close proximity. I felt that water in the creek from run off would have pesticides in it because of the farms, thus the bacteria near the creek would be exposed to pesticides.
McGovern Centennial Gardens at Hermann Park was chosen for soil sampling because it is a community garden in which consumable products are grown. The nature of the produce being grown and the location were also factors that were taken into consideration.
Soil sample was collected from irrigation ditches in a flower bed.
Over the course of a few weeks, it was evident that no result was going to be obtained for MP. By the 5th week, all tubes were close to becoming clear with a very slight yellow color.
The isolated bacteria degraded OP compound(s) on both MP & Px Media
The soil sample was actually hard like clay and was very dark when I had collected it from the park
Approx. 10–20 grams of soil was collected at a depth of approx. 20–30 cm from a grass farm in Crosby, TX on January 24, 2016. The sample was stored in an opaque 50 mL tube and kept at room temperature (approx. 25ºC). The soil at the collection site was wet after a day of rain.
Sample taken from Memorial Park Golf Course located at 6051 Memorial Dr. Houston, TX 77007. U.S.
This sample was taken from a garden in a residential area near community tennis courts in Misty Meadow Ln, Houston, TX.
The soil sample presented OP degradation
The soil sample was collected from a residential ranch in Dayton, Texas that used to have an area for vegetation growth.
My soil sample long description - about how and where the sample was taken and any special circumstances
Sample Collected near at the gated boardwalk area near the ship since the other side was closed off closer to the waters. Highly polluted area(cigarettes, paper, trash, food, etc)
Soil Collection from the Location: Pasadena Refining System, Inc located at the Houston Ship Channel. 29°43'07.3"N 95°12'37.5"
Soil sample from River Green Park in Katy, Texas (10 inches deep). The soil is very moisture and dark.
Soil sample collected near the San Jacinto river.
The location was in Buffalo Bayou between Sam Houston Tollway and Independence Pkwy. The soil was collected near the Old River along Launch Rd (Old River Terrace, Channelview TX). The approximate latitude and longitude coordinates are: 29°46'54.2"N 95°06'03.3"W. The soil was collected on August 30, 2016 around 9:30 PM. The collected sample was clay-like and moist because of the recent rainy weather.
The sample had bacteria that were able to degrade organphosphorous hydrolase found in Paraoxon pesticide
Soil was collected at a recreational park where kids play and people exercise. There is also a dog park.
The Soil Sample was collected in Channel View river bank by a public park around 5:27pm at 8/26/2016. The longitude and latitude was 29°46’57”N 96°6’9”W. The soil consistency was dark grey and very clay-like.
This soil sample was collected at Four Seasons Park near Katy area. The time was around 5:00pm on August 30, 2016. The latitude is N 29°50'25.956'' and the longitude is W 95°48'2406''.
It was obtained from a muddy region by the technology annex building
After 5 weeks of testing by progressively diluting the bacteria sample in SSM (Sulfur selective media) and CSM (Carbon Selective media) in both Methyl Parathion and Paraoxon condition it was determined what type organophosphatase activity is found in this bacteria sample after growth in a media rich dish. SSM consistently had more turbidity than the CSM (Carbon selective media) , but only the PX (paraoxon) samples ever had a breakdown to form the yellow color p-nitrophenol product in both, turbidity or no; MP, or Methyl Parathion media NEVER had yellow coloration. This trend stayed throughout, but eventually only the CSM had a yellow color in the 5th week trial with almost no turbidity in any sample. The final trial's SSM PX should still have had a yellow color, but it may have been due to a too great dilution of the soil bacteria by this point that it could not break down the Paraoxon, whereas the CSM-PX had just enough to break down the Paraoxon. However, my bacteria in particular grew best on an SSM based on turbidity consistently staying higher in the SSM tubes. This means that it is a bacteria that breaks down Paraoxon in order to obtain its sulfur or carbon products; the slightly higher level of turbidity in the SSM media throughout the 5 weeks may indicate the bacteria prefers sulfur for growth.
Soil collected from the banks of McGovern Lake outside of the Houston Zoo.
The bacteria collected were inoculated in both Methyl Parathion and Paraoxon for 5 weeks after allowing it to grow in a LB broth for a week. The Methyl Parathion samples showed no signs of life by week 3, but the sample was able to grow and digest Paraoxon. Turbidity was observed in all of the sample tubes for the first 2 weeks.
Soil sample was collected on the banks of the Buffalo Bayou. There were men fishing and a great deal of trash (beer cans, used condoms, etc) in the area. Dug about 10 inches down and collected sample in an amber tube and kept at room temperature.
Got soil from buffalo bayou, by the battle of Texas Historic Site
Soil collected from Channelview TX off of Market street near the bank of the San Jacinto River
Ballester Fishing Area - La Porte
Muddy sample of soil
Tolerant of Paraoxon. Intolerant of methyl parathion. 2 paraoxon hydrolyzing colonies.
Bacteria tolerant to PX. Several colonies showed halos around the edges which means there is a presence of hydrolysis of PX.
Collected in Sugar Land, TX. Degradation of Bacteria on OP Compound Paraoxon
Sample taken from bank of San Jacinto river near market street.
The sample was taken from cullinan park in January after a stormy day when the soil was still wet.
Very tiny colonies grew on the agar (~8478), but there was no evidence of Px hydrolysis based on the edges of the bacterial colonies. This means that the bacteria can tolerate the Paraoxon pesticide, but not degrade it.
The soil sample was taken taken from a local park located in SugarLand. A 8-10 feet hole was dug into the ground and a mixture of wet and dry soil was collected in put into the soil collection tube. Over the course of 5 weeks it was noted that the Sulfur Selective Media with the Paraoxon was the best at showing pesticide degradation in bacteria. It was noted in week 4 and week 5 of the experiment that the the SSM+PX tube turned orange then, burnt orange. In the agar plate, it was noted that the PX resulted in two types of bacteria, the larger bacteria around the outside was able to hydrolyze and the bacteria towards the center was only able to tolerate the paraoxon.
There was growth on the plate and few halos, so it is deduced that the bacteria is able to tolerate the pesticide and not necessarily degrade it. This could be evidence that a degrading bacteria was missed along the process.
(Student: Alyssa Nguyen) Soil sample taken near bank of Buffalo Bayou after Hurricane Harvey - Dug about 5 inches. Had a lot of clay when obtained.
Sample was collected to test for hydrolysis of op compound
Sample was taken after Hurricane Harvey at Buffalo Bayou. Soil Sample was mostly wet sand/dirt mixture.
Sample collected from a little away from a natural gas plant. Just on the outter edges of an agricultural field.
Collected the bank of Greens Bayou near the underpass of the Green River Drive bridge. Hurricane Harvey had hit Houston recently.
20g of soil were obtained from Alex's garden. Soil has been exposed to pesticides for at least 12 months.
Sample was taken from behind a home garage because it was suspected to have been treated for ants with strong chemical insecticides.
Sample was taken from a small cotton farm that used pesticides. Bacteria was found to tolerate Paraoxon but not degrade it.
Sample was collected from a small plot of soil used communicably by the apartment complex residents.
one of the agar plates has too many colonies therefore it was difficult to count but the second agar plate has many small colonies. the fuzzyiness around the colonies means degrading.
Old Richmond Rd and Copano Bay Drive. It came from a little flower patch.
CSM with Paraoxion was yellow in color, had some growth when plated on CSM agar, tested positive
Soil collected from a former cotton farm in Rosenberg, TX.
First Paraoxon Plating 88 was at a 1:100 200ul sample Second Paraoxon Plating 16 was at a 1:1000 200ul sample
The soil sample was collected on September 5, 2012. It was continuously stored at room temperature.
Soil sample collected from backyard (side of the house near the main water line). Coordinates: N 29°40.058' W095°35.364' Even though there were signs of pesticide degradation detected in media with Paraoxon (Px) pesticide present, sample did not grow after inoculation into enriched media and overnight incubation. Sample was a false positive.
Sample was collected 8 feet from the fence line.
My sample was collected from the front lawn of the home at address 1017 Brookhollow Drive, Deer Park, TX 77536; my place of residence. The city of Deer Park has trucks spray pesticides meant to control mosquitoes every Mon-Thur during the mosquito season. This practice has been in use since the late 80's, and could have helped with the creation of pesticide-degrading bacteria.
The sample was collected from a ditch on the corner of Liberty Dr and Julie St. Mosquito trucks drive by and spray pesticide every week during the summer months.
Negative results for paraxoan and methyl parathion digesting bacteria.
Paraoxon positive, MP negative
CSM with paraoxon was yellow. Growth halo and tested positive. There was about 14 colonies on the plate
this sample was collected from a bayou with the adress of 16600-17080 Beechnut st Houston TX 77083 on 09/08/2012. the sample was moist.
The sample is collected from cotton field. The sample was positive for Paraoxon and negative for Methyl parathion.
Soil sample was collected at 9:10 AM on August 27, 2010
Px CSM Weeks 1-5: CYYYY Px SSM Weeks 1-5: WWTTT MP CSM Weeks 1-5: SSSSS MP SSM Weeks 1-5: WWTTT Plated on CSM agar with PX and MP. Growth with halo in both PX and MP. Preliminary Hydrolysis Test was Positive in both PX and MP. Location on Farm to Market Road 521 (approximate address 11730) in between and parallel to Willow Lake and County Road 55. Coordinates: 29.413507,-95.477886
Sample taken from a crop field currently in use in Alvin, TX.
Sample taken from rural area in South Houston on FM 2234. Exhibited a positive reaction with Px + SSM. Showed evidence of OP hydrolysis.
Collected in or near an area that was cultivated for rice 50 years ago.
SAMPLE WAS WET AND THE FARM WAS IN OPERATION. THEY GREW FRUITS AND VEGETABLES AND SOLD TO CUSTOMERS.
Soil sample taken from Froberg's Farms.
Soil sample collected from the strawberry field at Froberg's Farm. Sample was taken at 9 am on September 21, 2012. Results over 5 weeks of isolationg and growing the bacteria from the sample. Paraoxon (PX)& Methyl Parathion (MP)are insecticides CSM and SSM are medium. Y = yellow, T= colorless, C = slight yellow and turbid, S=slight yellow and clear, W=turbid white W# is week number PX + CSM = W1-Y, W2-Y, W3-Y, W4-Y, W5-Y PX + SSM = W1-W, W2-T, W3-S, W4-Y, W5-S MP + CSM = W1-W, W2-T, W3-T, W4-T, W5-T MP + SSM = W1-C, W2-C, W3-T, W4-T, W5-T Around 15 colonies of Paraoxon grew on CSM agar plate but no halo was showed on colony.
Paraoxon hydrolyzing bacteria are present. Showed negative results for Methyl Parathion degradation.
Soil was taken from a strawberry farm, the soil was light brown and slightly dry.
The sample was taken from the soil in a front yard in South Houston. The sample tested positive for Paraoxon, producing tolerant colonies.
The soil was light brown and dry.
Sample was collected from field near North Main Street, Pearland TX. Although there was a positive result for paraoxon hydrolysis, there was no growth when added into the enriched media and incubated. There were a lack of microorganisms for the sample. Coordinates: 29° 35’ 30’’ N 95° 17’ 12’’ W
Kevin Smith sample 1
Week 1 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. Y C N N N N Week 2 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. W W W W W W Week 3 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T T S T T Week 4 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T W S T T Week 5 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T T S T S
The soil sample came out to be positive for paraoxon and was able to withstand the pesticide.
This sample was collected from the nearby shore of Rio Grande, the main river that divides the US with Mexico along Texas. It is noted that a significant amount of contaminants run down this river, making it a very suitable place for data collection. The sample was taken from the US side in a nearby zone close to the international Bridge Juarez Lincoln. During the incubation and transferring process of 5 weeks, the sample displayed increased activity in Paraoxon CSM enriched media with a noticeable yellow coloration, whereas the rest of the tubes did not show any activity and progressively faded into a complete clear coloration. After plating and growth the whole plate was completely covered and made colony count not possible, however prominent white/yellow colonies can be seen which are approximately 200. These colonies are possible zones of hydrolysis of bacteria that is expressing an OPH gene.
An unofficial count of the colonies yields an estimation of about 79 total colonies, with 51 OP degrading colonies, and 28 OP tolerant colonies.
Sample was taken from a residential that have been treated for ants with strong chemical insecticides.The agar plates has a large number of very small colonies.
The soil was collected from farmland in La Porte, Texas where many crops have been grown. I dug a hole approximately 8 inches deep to collect the soil and kept the soil in an amber tube at room temperature to avoid the exposure of sunlight and heat.
The sample was taken from the backyard near the trunk of a tree.
A soil sample was collected near a guava tree from a residential backyard. The sample was positive for paraoxon tolerance.
A small soil sample was collected on a plot of farming land on Fernglade Drive near 4033 Gladeridge Dr, Houston, TX 77068.
testing soil sample to see if it has signs of pesticide degrading bacteria in it.
Sample tested positive form paraoxon with about 26 white colonies. Halos are present which show sign of degradation.
Week 1 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-Yellow Mp SSM-Slight Yellow Week 2 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Slight Yellow Week 3 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White Week 4 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White Week 5 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White
Date: September 21, 2011 Week 1 Paraoxon (Px)+ CSM = So Paraoxon (Px)+ SSM = So Methyl Parathion (MP) + CSM = So Methyl Parathion (MP) + SSM = W Date: September 28, 2011 WEEK 2 Paraoxon (Px)+ CSM = Y Paraoxon (Px)+ SSM = S Methyl Parathion (MP) + CSM = S Methyl Parathion (MP) + SSM = W Date: October 05, 2011 WEEK 3 Paraoxon (Px)+ CSM = C Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = S Date: October 12, 2011 WEEK 4 Paraoxon (Px)+ CSM = T Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = C Date: October 19, 2011 WEEK 5 Paraoxon (Px)+ CSM = Y Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = C Legend So- same color as soil inoculate W- turbid white T- colorless Y- Pronounced yellow color S- Slight yellow color C- Yellow color and lightly turbid
Digging a hole 12 inches into the ground and obtaining the soil in a sample container tube.
Grew well when inoculated with CSM Paraoxon. Plated on CSM agar with Paraoxon. Plating results showed growth with halo. Bacteria sample tested positive for Preliminary Hydrolysis Test.
The sample was acquired from a private church garden in Northwest Houston from an area that had recently been planted with corn and other harvest crops nearby. The sample was positive for Paraoxon tolerance and was able to degrade (hydrolyze) the pesticide and OP compounds.
A sample of soil was collected from Cimarron Elementary. It was tested for OP degrading bacteria and produced negative results.
My sample was positive for Paraoxon but negative for Methyl Parathion. In the 5 week subculturing leading up to platting there was reason to believe that there was degradation of Paraoxon and Methyl Parathion till week 3 when all yellow color faded from the Methyl Parathion tubes.
Soil Sample was collected from a farm outside Rosenberg. The farm seemed freshly sown because the soil was turned over and some crop seem to have been planted.
The soil was collected from a flower bed. The soil was soft and moist.
This sample was taken from the Walmart garden center, where they house plants and other botanical forestry
Soil collected at a soccer field in southwest Houston.
Sample was more like a mud texture, heavy rains over Memorial Day weekend. The sample was collected about 10 yards away from actual bayou water and about 8 inches below surface. It was moist, not too wet neither too dry. Its smell was very peculiar. Paraoxon (Px) Methyl Parathion (MP) CSM SSM CSM SSM WEEK 1 N/A N/A N/A N/A WEEK 2 slight yellow and turbid pronounce yellow and clear slight yellow and clear turbid white WEEK 3 slight yellow and turbid pronounce yellow and clear slight yellow and clear colorless and clear WEEK 4 slight yellow and turbid pronounce yellow and clear turbid white colorless and clear WEEK 5 slight yellow and turbid pronounce yellow and clear turbid white colorless and clear At time of screening for OP hydrolysis, there was no growth.
Soil sample collected from the bayou behind the apartment complex Bayou Oaks from University of Houston Housing. Sample collected from 12 inches deep ground for better chances of getting a better result.
Approximately 15 grams of moist Soil Sample was collected by the Miller Outdoor Theatre.
Soil sample from front yard in a farm house (10 inches depth). the soil is very moisture and dark.
The cafe has a garden behind the shop. This sample is collected from that location
A hole was was dug approximately 12 inches deep, right off the trail, about 8 feet from the bayou water, and approximately 20-gram soil sample was collected, then placed in a 50mL amber tube. The soil sample was a mixture of moist and dried dirt.
An 12 inches hole was dig and soil was obtain. The soil sample was first inoculated in an enriched media and incubate at 37 degree Celsius. The soil containing media was then pipettes in minimal medical containing paraoxon and methyl parathion. Again was incubate for 7 day at 37 degree Celsius. This process was repeated until week 5. Then plated onto minimal agar with pesticide to observe for OP hydrolysis.
Soil was collected from Mason Park near a marsh. After liquid media culturing and screening on a minimal agar plate, the sample was found to be Paraoxon Positive and Methyl Parathion Negative.
Sample taken from landscaped garden plot near fountain in Buffalo Bayou Park.
Soil collected from a golf course
This garden grew crops the family ate from daily so it is very likely that they used very minimal pesticides to treat their soil. Sample was moist and dark and collected on a sunny day at approximately 11:30 AM. Sample was not further processed due to lack of evidence of strong hydrolysis.
A sample was taken from a home garden. it was then tested to see if pesticide degrading enzyme were found.
Very clay like, thick and adhesive. Plenty of moisture
Mound of dirt collected from about 3-6 inches deep near
Soil Sample was taken from Blessington Farm. Originally thought to be laced with pesticides, but found out from the owners that minimal pesticides were used on their farms. Additionally, flood water submerged the farm back in April might had an impact on the bacteria community. The spot chosen on the farm was known to be with "most" pesticides, the soil collected was clay-like and smell like manures.
Sample taken from a park that had water from the Old River, that connects to the San Jacinto River.
The soil sample was collected from Texas Avenue Park located in Webster, Texas. The sample was put into tubes containing media with two different organophosphorus compounds, Methyl Parathion and Paraoxon. The microorganisms were able to degrade Paraoxon, but not Methyl Parathion.
Clay like texture to the soil and it was wet
Sample taken from a Garden Center which use pesticides in all their plants.
Muddy sample along the waters edge
Soil sample with unknown composition
Muddy soil sample with unknown composition
Muddy sample with unknown composition
Muddy soil sample with unknown components.
Muddy soil sample
Muddy soil sample
A large cell clump covering in the plate. Some hydrolysis is presented on the sample. No Methyl Paraxon growth was presented on the plate.
Bacteria collected and cultivated.
I got my sample from a bayou by the Fort Bend County fairground in Rosenberg.
Soil sample of about 10-20 grams found behind house, in a bayou that gets flooded occasionally.
Sample taken from the edge of the White Lake in Cullinan Park.
This sample was collected from farmland, which was thought to contain OP hydrolyzing bacteria. The bacteria isolated from the sample, which was collected during the spring 2017 semester, was paraoxon tolerant but not capable of OP hydrolysis.
sample was from the buffalo bayou area
Sample positive for paraoxon hydrolysis. Sample negative for methyl parathion hydrolysis
Charles Milby park and Sims Bayou is down stream from Buffalo Bayou, where PCI Nitrogen - a fertilizer production facility. The sample bacteria were able to hydrolyze Paraoxon in the sulfur-selected medium after five weeks of dilution, transfer, and incubation.
Many small colonies. No hydrolysis is presented on the sample. No Methyl Parathion growth was presented on the plate.
Retrieved near Brazos River
sample collected along sidewalk of Sabine Park, after Hurricane Harvey Flood.
The sample was a mixture of sand and dirt. It was taken from an area that was affected by flood water. Once grown on a Px plate there was many tolerate bacteria and a few bacteria colonies that degraded the pesticide with soft edges.
Sample was milk chocolate brown, with some moisture present.
Soil collected from hole approximately 10 inches deep three days after flood waters receded.
The sample was mostly solid with a little bit of twigs and sand mixed in.
Sample was collected after hurricane Harvey when it was still very wet.
Dirt was retrieved on a cliff near the river, the dirt was moist since it had rained a day or two before it was collected.
Soil was collected from park close to University of Houston-Main Campus
The soil sample was dug from an area close to the big lake of the Kickerillo-Mischer Preserve in Houston. There was dead fish all over the margins of the lake because Harvey had brought fish from other areas (farms) that were not surviving to that particular environment.
Moist and dense soil extracted from Bayland Park Dr near a baseball field from an ~8 inch deep hole in the ground.
The sample was taken from Buffalo Bayou Park after heavy rainfall.
Soil collection from the Brazos river near a recreational park on a rainy day. Sample tolerated well in minimal medium but demonstrated no sight of hydrolysis.
the sample was collected near the river.
This soil sample was taken from within the Barker Reservoir after Hurricane Harvey.
Sample was collected by the banks of the buffalo bayou. Because of the recent hurricane Harvey, the bayou was overflowed and some soil might have been washed out.
CSM MP results gave positive results from the beginning about degradation. CSM PX began to show slight degradation around the week 3 of sample transfer. Slight halo effects on the edges of the plate indicates OP hydrolysis has taken place. SSM MP and PX have shown negative results of degradation from the initial stage. In comparison to other members, I did not have any positive results for both SSM Mp/ Px.
I collected a sample that was at the waters edge where many birds had been so the top layer was mostly white bird poop and the lower levels were clay with colorful bands of sand and a little bit of algae that was floating atop it. It smelled like Galveston beach, which is ironic (or not) because Bray's Bayou flows right into Galveston Bay.
Soil was collected near a sewer in a ditch behind HEB. The bacteria were found to be tolerating the paraoxon pesticide.
Soil sample collection plated on CSM agar for the viewing and identification of degrading organophosphorus bacteria.
The soil obtained was in my parent's garden.
The soil sample had a little moisture content when collected from the farm.
My sample contains approximately 80-95 positive tolerant colonies. The tolerant bacterial colonies weakly or incompletely degraded CSM media containing Paraoxon pesticide to grow and survive.
Soil sample was collected from a cotton farmland located in the Rosenberg area of Texas.
The location from where the sample was taken from had previous exposure to pesticides, and was taken from the backyard of a garden in the Northwest Houston Area.
My sample appears to be from farm land. My starting sample was not as soft as other student's samples. The soil I collected had a texture more like clay than regular soil.
The sample was collected from a strawberry farm in Fresno, TX. The sample tested positive for Paraxon and yielded tolerant colonies.
The soil sample was collected from a park. The sample was tested postivite for Paraxon and bacteria tolerated the toxic environmnet.
This sample contains bacteria capable of hydrolyzing Paraoxon(Px) present in pesticides. After the transfer of 100microliters into MM plate, a total count of approximately 540 colonies was observed. Number of Tolerant colonies: approximately 530 Number of Degrading colonies: approximately 10
The colonies were grown on CSM agar + Paraoxon(100mg/mL conc.) @ 1uL/mL Notes 1:100 Dilution (in SOC, 200uL plated) (in SOC, 200uL plated) 11 colonies 1:1000 Dilution (in SOC, 200uL plated) (in SOC, 200uL plated) 2 colonies Total: 13 colonies
sample taken from a Landscaping, Nursery and Tree farm in Pasadena Texas.
Grayish Brown and dry soil sample. It was collected from the land where it was farmland before.
This is a community farm where pesticide is not used. Approximately 100 bacterial colonies growth seen on agar plate with Paraoxon. Half of them appeared white with a halo ring in the surrounding and the other half appeared white. There is indication of bacteria that tolerate Paraoxon and bacteria that degrade paraoxon in the soil sample.
Sample collected from 1620 N Houston Ave, Humble, TX 77338. Some colonies were very large in diameter compared to other students' results. The ring of hydrolysis is very small.
Brown clumpy soil. no scent. Citrus fruit. 1 foot beneath the surface.
The collected soil was from Froberg Farm, where several vegetables along with strawberries are grown. The soil was dark brown in color and heavily moistened. During the process of isolating and growing the bacteria present in the soil sample, I have observed color changing patterns in a weekly basis on specific combination of medium and insecticide. The results are as follows: Week 1, 9/21/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = yellow color and lightly turbid, (C) Methyl Parathion (MP) + CSM = pronounce yellow color (Y) Methyl Parathion (MP) + SSM = yellow color and lightly turbid, (C) Week 2, 9/28/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 3, 10/05/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 4, 10/12/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 5, 10/19/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Around 80-90 colonies of bacteria were successfully isolated from the soil sample and grown well on the CSM Agar media with Paraoxon pesticide
sample tested positive for CSM-Px pesticide.
Collection of soil sample to test for pesticides, It was collected from my garden where I grew carnations.
I collected this soil from the cotton field. I thought this would be great idea because cotton field is one of the farms where farmers use lots of pesticides and insecticides.
My soil sample was tested for OP biodegradable bacteria. The pesticide used was paraoxon. Testing was done within a 5 week period and results of the tests were negative.
The sample was obtained near the Sweetwater Cypress Golf Course on September 3rd, 2012.
The sample was taken in the backyard, near the trunk of a tree.
The sample was dry and clumpy dirt resembling nestle chocolate powder. It was collected from Theiss Farms. The sample tested negative for Organophosphorus degradation.
The soil sample was collected from a vegetable garden in my backyard. The soil sample was dry and crumbly. The results were negative for degrading bacteria.
Sample was collected from my home garden, which is treated with pesticides every 3 months. Regardless, no evidence of pesticide degrading or tolerant bacteria was found.
Sample taken from garden with hopes of finding organophosphorous degrading bacteria
Soil collected from JRN Nursery.
The MP samples did not show strong evidence of hydrolysis at all. MP plates did not show any growth 29°54'49.1"N 95°42'13.3"W
The soil sample was collected from garden at home that has frequent pesticides
The soil was collected at the Kingwood vegetable garden in Kingwood TX.
Soil collected from the Brock Park Municipal Golf Course
taken from bear creek park in Houston, Tx, soil taken from approximately 12 inches into the ground,
Portico at West 8 apartments
This sample was collected from a farm with freshly sewed soil from Rosenburg, Tx.
sample was collected about 10 yards from the receding water line, water of the bayou was about at 70% capacity during the time of collection because of a series of heavy rain falls from the weeks leading up to the collection which included a heavy flooding during memorial day weekend 2015. This meant that the location of collection was resting in standing water.
front yard of a house in farming area in Jersey Village (Houston, Texas). 8 inches deep. The sample looks dark and moisture when i got it fromt he yard.
Soil collect from Shadow Hawk Golf Club
Soil was extracted from ~10" under ground.
This site is near Greens Bayou, which was contaminated with DDT. There was a concrete slab inside plastic fabrication plant, upstream, that had stains from liquid dripping into the ditch. Likely from rain, but may also have carried contaminants from the plant into the ditch. Sample was taken from this ditch close to the drain by a back gate, see attached image. Sample was taken halfway down; despite being above the water table below sample was mostly mud.
Sample collected at the surface, dry and the land was not used to grow anything. Did not expect to find pesticide degrading bacteria.
Soil was collected in a backyard in a suburb in Killeen, Texas. The soil was collected close to the house as the house gets regularly sprayed for bugs.
Soil was collected from a flower bed in Cypress, TX 77433
By the memorial swimming pool, in front of a tree. The dirt was collected from 10 inches deep. Dirt was semi-moist and dark in color.
Soil sample was collected from brays bayou biking trail. It was collected around 2:30 AM. There was a 12 inch deep hole digged to retrieve it.
The soil was collected from Steinhagen Farms on crop growing fields. After many weeks of sampling and screening on minimal media; the sample was found to be Paraxon Positive; and Methyl Parathion Negative (yellow).
Sample was taken from John P. McGovern Centennial Gardens in Houston, TX.
Collected in a home garden.
Soil sample obtain from a golf course nearby cinco ranch katy tx
Sample was collected from a backyard garden. The area had 15 years of previous pesticide usage.
Soil collected from a home garden and pesticides are used consistently in this garden. 10g of Soil sample was collected into 50mL amber tube
was wet, clumped, and muddy.
Sample was obtained at a home garden it showed Paraoxon tolerance.
The sample was mostly dry, but some parts were wet. The sample showed no sign of hydrolysis therefore was not inoculated into rich media and was not plated onto a plate
Soil collected from a home garden.
The bacteria sample collected was able to degrade the PX pesticide, but was unable to degrade the MP pesticide
sample was obtained from golf Course which resulted in dgredgation of OP
Positive for Paraoxon and negative for Methly Parthion
The soil sample was collected from the small garden where usually use pesticide
Growth of pesticide resisting bacteria colonies in Paraoxon and Methyl Parathion. In Paraoxon plate degrading bacteria is present. In Methyl Parathion plate tolerating bacteria is present.
Was clumpy, wet soil from a ricefield.
The soil sample was collected from River Terrace Park on Lakeside Drive in Channelview, Texas.
The soil sample was dark grey, sandy and smelled like gasoline/crude oil.
Soil was just a brown color, with a bit of a red tint. Did not really smell out of the ordinary, just smelled like regular dirt.
Soil sample was collected on the banks of the Buffalo Bayou. There were men fishing and a great deal of trash (beer cans, used condoms, etc) in the area.
This sample was collected in a public park by a bank of along the San Jacinto River
The soil sample was collected on August 27, 2016 from Atkinson Farms. The plated sample displayed positive growth after exposure to Paraoxon and negative growth after exposure to Methyl Parathion.
The soil sample was collected from the side of Berry Bayou. A hole was dug, 6-8 inches deep, and the soil at the bottom of the hole was collected and stored into a dark tube.The sample was kept at room temperature.
Soil Sample: Sandy soil slightly moist from the bank of the bayou located inside of the park with debris spread within.
PX and MP screening for OP hydrolysis
Soil Sample from Mary Jo Peckham Park in Katy, Texas.
Sample was taken from a 3 acre farm that has been in use as farm land since 2012.
The soil sample was collected in a drainage ditch in the Katy area
This sample was collected near a drain at Cullen park which is a part of Addicks Reservoir, after Hurricane Harvey
Park is regularly treated with pesticides and other chemical agents.
moist soil off the banks of Willow Waterhole Bayou offshoot, clumpy and clay-like
The soil sample was taken from dirt that was slightly wet due to rain the previous day. The soil's location is near a river source, but also near a residential area.
Soil sample collected from a bank on the San Jacinto River Bay near the Fred Hartman Bridge. Spring 2018
soil collection from Bayou Parkland near Brays Bayou
This soil sample was collected from the banks of Brays Bayou that recently might have overflowed due to Hurricane Harvey.
This sample was collected near a small body of water which was flooded during Hurricane Harvey. The sample was Px positive in both CSM and SSM and negative in both MP medias.
Sample was collected from the edge of a lake between a suburban and museum of natural sciences.
Found near a residential man-made pond.
Soil collected from a small runoff from Brays Bayou located on Chimney Rock near South Braeswood.
Sample was top layer of clay and middle layer of sand. The sample was also very muddy.
It was muddy, and wet.
Sample was collected from the banks of Brays Bayou next to the Brays Bayou Greenway Trial. The sample was dug up from about 6-8 Inches deep. The sample was collected after a flood therefore the soil sample was very damp and had the texture of clay.
Found near a gulf course by the Steep Bank Creek
Soil sample was collected at the banks of the Buffalo Bayou in Houston Tx. Because of the recent hurricane Harvey, the bayou overflowed, washing out some soil. This may have an impact in the results of the experiment.
sample was taken under bridge in the middle of Steep Bank Creek
Soil sample collected near the Historic Santa Anna Capture Site
Soil collected from Tom Bass Regional Park near Clear Creek.
Soil Collected from Sugarland Park NN